SMALP Publications and Patents

The following papers report significant developments in the SMALP field. We invite you to submit a title for a research article to be published in a special issue of BBA Biomembranes on Membrane Proteins: Novel Targets and Technologies that we're organizing in 2019.


Overduin M, Esmaili M. Structures and Interactions of Transmembrane Targets in Native Nanodiscs. SLAS Discovery. in press.

Kehlenbeck DM, Josts I, Nitsche J, Busch S, Forsyth VT, Tidow H. Comparison of lipidic carrier systems for integral membrane proteins - MsbA as case study. Biol Chem, in press.

Redhair M, Clouser AF, Atkins WM. Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs. Chem Phys Lipids. 220:14-22

Pollock NL, Rai M, Simon KS, Hesketh SJ, Teo ACK, Parmar M, Sridhar P, Collins R, Lee SC, Stroud ZN, Bakker SE, Muench SP, Barton CH, Hurlbut G, Roper DI, Smith CJI, Knowles TJ, Spickett CM, East JM, Postis V, Dafforn TR. SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis. BBA Biomembr. 1861(8):1437-1445.

Bada Juarez JF, O'Rourke D, Judge PJ, Liu LC, Hodgkin J, Watts A. Lipodisqs for eukaryote lipidomics with retention of viability: Sensitivity and resistance to Leucobacter infection linked to C.elegans cuticle composition. Chem Phys Lipids. 222:51-58

Overduin M, Esmaili M. Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics. Appl. Sci. 2019, 9(6), 1230.

Ognjenović J, Grisshammer R, Subramaniam S. Frontiers in Cryo Electron Microscopy of Complex Macromolecular Assemblies. Annu Rev Biomed Eng. in press.

M Yokogawa, M Fukuda, M Osawa Nanodiscs for Structural Biology in a Membranous Environment. Chem Pharm Bull. 67 321-326.

Á Deák, D Sebők, E Csapó, A Bérczi, I Dékány, Zimányi L, Janovák L. Evaluation of pH-responsive poly (styrene-co-maleic acid) copolymer nanoparticles for the encapsulation and pH-dependent release of ketoprofen and and tocopherol model drugs. Eur Polymer J. 114, 361-368

Pinwanich P, Soisungval, A.Structural Modification of Styrene Maleic Anhydride Copolymers for Plant Bioactive Compound Extraction, Key Engineering Materials 798, 351-357.

Sahoo BR, Genjo T, Moharana KC, Ramamoorthy A. Self-Assembly of Polymer-Encased Lipid Nanodiscs and Membrane Protein Reconstitution. J Phys Chem B. in press.

Hortigüela V, Larrañaga E, Lagunas A, Acosta GA, Albericio F, Andilla J, Loza-Alvarez P, Martínez E. Large -Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies. Nanomaterials (Basel). 9(4). pii: E579

Sun C, Gennis RB. Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs. Chem Phys Lipids. 221:114-119

Mosslehy W, Voskoboynikova N, Colbasevici A, Ricke A, Klose D, Klare JP, Mulkidzhanyan AY, Steinhoff HJ. Conformational dynamics of sensory rhodopsin II in nanolipoprotein and styrene-maleic acid lipid particles. Photochem Photobiol. in press.

Danielczak B, Meister A, Keller S. Influence of Mg(2+) and Ca(2+) on Nanodisc Formation by Diisobutylene/Maleic Acid (DIBMA) Copolymer. Chem Phys Lipids. in press.

Kopf AH, Koorengevela MC, van Walreea CA, Dafforn TR, Killian A. A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers. Chem Phys Lipids. in press.

Sahoo BR, Genjo T, Nakayama TW, Stoddard AK, Ando T, Yasuhara K, Fierke CA, Ramamoorthy A. A cationic polymethacrylate-copolymer acts as an agonist for β-amyloid and an antagonist for amylin fibrillation, Royal Soc Chem, in press.

Bada Juarez JF, Harper AJ, Judge PJ, Tonge SR, Watts A. From polymer chemistry to structural biology: The development of SMA and related amphipathic polymers for membrane protein extraction and solubilisation. Chem Phys Lipids. 221:167-175.

Dathe A, Heitkamp T, Pérez I, Sielaff H, Westphal A, Reuter S, Mrowka R, Börsch M.Observing monomer - dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap. arXiv 1902.01511

Prakash P, Litwin D, Liang H, Sarkar-Banerjee S, Dolino D, Zhou Y, Hancock JF, Jayaraman V, Gorfe AA. Dynamics of membrane-bound G12V-KRAS from simulations and single-molecule FRET in native nanodiscs. Biophys J.116(2):179-183.

Zhang TR, Wang CX, Dong FQ, Gao ZY, Zhang CJ, Zhang XJ, Fu LM, Wang Y, Zhang JP. Uptake and translocation of styrene maleic anhydride nanoparticles in Murraya exotica plants as revealed by noninvasive, real-time optical bioimaging. Environ Sci Technol. 53(3):1471-1481.

Redhair M, Clouser AF, Atkins WM. Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs. Chem Phys Lipids 220:14-22.

Bali AP, Sahu ID, Craig AF, Clark EE, Burridge KM, Dolan MT, Dabney-Smith C, Konkolewicz D, Lorigan GA. Structural Characterization of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using EPR spectroscopy. Chem Phys Lipids. 220:6-13.

Domínguez Pardo JJ, van Walree CA, Egmond MR, Koorengevel MC, Killian JA. Nanodiscs bounded by styrene-maleic acid allow trans-cis isomerization of enclosed photoswitches of azobenzene labeled lipids. Chem Phys Lipids 220:1-5.

Ravula T, Hardin NZ, Ramamoorthy A. Polymer nanodiscs: advantages and limitations. Chem Phys Lipids 219:45-49.

Bassard JE, Laursen T, Molecular snapshots of dynamic membrane-bound metabolons. Methods in Enzymology 167, 1-27.

Karlova MG, Voskoboynikova N, Gluhov GS, Abramochkin D, Malak OA, Mulkidzhanyan A, Loussouarn G, Steinhoff HJ, Shaitan KV, Sokolova OS. Detergent-free solubilization of human Kv channels expressed in mammalian cells. Chem Phys Lipids. 219:50-57.

Teo ACK, Lee SC, Pollock NL, Stroud Z, Hall S, Thakker A, Pitt AR, Dafforn TR, Spickett CM, Roper DI. Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein. Sci Rep. 9(1):1813.

Orekhov PS, Bozdaganyan M, Voskoboynikova N, Mulkidjanian AY, Steinhoff HJ, Shaitan KV. Styrene-maleic acid copolymers form SMALPs by pulling lipid patches out of the lipid bilayer. Langmuir 35(10):3748-3758

Salnikov ES, Aisenbrey C, Anantharamaiah GM, Bechinger B. Solid-state NMR structural investigations of peptide-based nanodiscs and of transmembrane helices in bicellar arrangements. Chem Phys Lipids 219:58-71.

Hardin NZ, Ravula T, Mauro GD, Ramamoorthy A. Hydrophobic functionalization of polyacrylic acid as a versatile platform for the development of polymer lipid nanodisks. Small 15(9):e1804813.

Keener JE, Zambrano DE, Zhang G, Zak CK, Reid DJ, Deodhar BS, Pemberton JE, Prell JS, Marty MT. Chemical additives enable native mass spectrometry measurement of membrane protein oligomeric state within intact nanodiscs. J Am Chem Soc, 141(2):1054-1061.

Harding BD, Dixit G, Burridge KM, Sahu ID, Dabney-Smith C, Edelmann RE, Konkolewicz D, Lorigan GA. Characterizing the structure of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for membrane protein spectroscopic studies. Chem Phys Lipids, 218:65-72.

Yeh, V. Study of Bacteriorhodopsin in a Controlled Lipid Environment Springer Thesis, pp 1-36.

Henrich E, Löhr F, Mezhyrova J, Laguerre A, Bernhard F, Dötsch V. Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments. Methods Enzymol. 2019;614:143-185

Yao Y, Marassi FM. Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs. Methods Mol Biol. 1877:233-246.

Overduin M & Esmaili M. Memtein: the fundamental unit of membrane:protein structure and function Chem Physics Lipids. 218:73-84.

Brady NG, Qian S, Bruce BD. Analysis of Styrene Maleic Acid Alternating Copolymer Supramolecular Assemblies in Solution by Small Angle X-Ray Scattering. Eur Polymer J. 111: 178-184.

Gulamhussein AA, Meah D, Soja DD, Fenner S, Saidani Z, Akram A, Lallie S, Mathews A, Painter C, Liddar MK, Mohammed Z, Chiu LK, Sumar SS, Healy H, lHussain N, Patel JH, Hall SC, Dafforn TR, Rothnie AJ. Examining the stability of membrane proteins within SMALPs. Eur Polymer J. 112: 120-125.

Korotych O, Mondal J, Gattás-Asfura KM, Hendricks J, Bruce BD. Evaluation of commercially available styrene-co-maleic acid polymers for the extraction of membrane proteins from spinach chloroplast thylakoids. Eur Polymer J. 114, 485-500.


Ravula T, Hardin NZ, Di Mauro GM, Ramamoorthy A. Styrene maleic acid derivates to enhance the applications of bio-inspired polymer based lipid-nanodiscs. Eur Polymer J. 108: 597-602.

Qiu W, Fu Z, Xu GG, Grassucci RA, Zhang Y, Frank J, Hendrickson WA, Guo Y. Structure and activity of lipid bilayer within a membrane-protein transporter. Proc Natl Acad Sci USA 115(51):12985-12990.

Harding BD, Dixit G, Burridge KM, Sahu ID, Dabney-Smith C, Edelmann RE, Konkolewicz D, Lorigan GA. Characterizing the structure of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for membrane protein spectroscopic studies. Chem Phys Lipids 218:65-72.

Simon KS, Pollock NL, Lee SC. Membrane protein nanoparticles: the shape of things to come. Biochem Soc Trans. 46(6):1495-1504.

Danielczak B, Keller S. Collisional lipid exchange among DIBMA-encapsulated nanodiscs (DIBMALPs). Eur Polymer J.109: 206-213.

Luna M, Vazir M, Vaish A, Chong S, Chen I, Yamane HK. Generation of membrane proteins in polymer-based lipoparticles as flow cytometry antigens Eur Polymer J. 109, 483-488.

Overduin M & Klumperman B. Advancing membrane biology with poly(styrene-co-maleic acid)-based native nanodiscs. Eur Polymer J. 110, 63-68

Hellwig N, Peetz O, Ahdash Z, Tascón I, Booth PJ, Mikusevic V, Diskowski M, Politis , Hellmich Y, Hänelt I, Reading E, Morgner N. Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs. Chem. Commun. 54, 13702-13705.

Barniol-Xicota M, Verhelst SHL. Stable and Functional Rhomboid Proteases in Lipid Nanodiscs by Using Diisobutylene/Maleic Acid Copolymers. J Am Chem Soc. 140(44):14557-14561.

Kopf AH, Koorengevel MC, van Walree CA, Dafforn TR, Killian JA. A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers. Chem Phys Lipids 218:85-90.

Schmidt V, Sidore M, Bechara C, Duneau JP, Sturgis JN. The lipid environment of Escherichia coli Aquaporin Z. Biochim Biophys Acta Biomembr. 1861(2):431-440.

Liu Y, Moura ECCM, Dörr JM, Scheidelaar S, Heger M, Egmond MR, Killian JA, Mohammadi T, Breukink E. Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers. PLoS One. 13(11):e0206692.

Lousa CdeM & Postis, V. Recent Advances on Polymer Lipid Particles (PoLP) in Membrane Protein Research, eLS, John Wiley & Sons, Ltd: Chicester.

Angelisová P, Ballek O, Sýkor J, Benad O, Čajk T, Pokorná J, Pinkas D, Hořejší, V. The use of styrene-maleic acid copolymer (SMA) for studies on T cell membrane rafts. BBA Biomembranes, 1861(1):130-141.

Ravula T, Hardin NZ, Bai J, Im SC, Waskell L, Ramamoorthy A. Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs. Chem Commun (Camb). 54(69):9615-9618.

Hall SCL, Tognoloni C, Charlton J, Bragginton ÉC, Rothnie AJ, Sridhar P, Wheatley M, Knowles TJ, Arnold T, Edler KJ, Dafforn TR. An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles. Nanoscale. 10(22):10609-10619.

Popot JL. Membrane Proteins in Aqueous Solutions Optical Spectroscopy of Membrane Protein/Amphipol Complexes, Biological and Medical Physics, Biomedical Engineering. 381-404

Thonghin N, Kargas V, Clews J, Ford RC. Cryo-electron microscopy of membrane proteins. BBA Methods. pii: S1046-2023(17)30374-2.

Sgro GG, Costa TRD. Cryo-EM Grid Preparation of Membrane Protein Samples for Single Particle Analysis. Front Mol Biosci. 5:74.

Mio K, Sato C. Lipid environment of membrane proteins in cryo-EM based structural analysis. Biophys Rev. 10(2):307-316.

Xue M, Cheng L, Faustino I, Guo W, Marrink SJ. Molecular Mechanism of Lipid Nanodisk Formation by Styrene-Maleic Acid Copolymers. Biophys J.115(3):494-502.

Domínguez Pardo JJ, Koorengevel MC, Uwugiaren N, Weijers J, Kopf AH, Jahn H, van Walree CA, van Steenbergen MJ, Killian JA. Membrane Solubilization by Styrene-Maleic Acid Copolymers: Delineating the Role of Polymer Length. Biophys J. 115(1):129-138.

Radoicic J, Park SH, Opella SJ. Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins. Biophys J. 115(1):22-25..

Sun C, Benlekbir S, Venkatakrishnan P, Wang Y, Hong S , Hosler J, Tajkhorshid E, Rubinstein JL & Gennis RB. Structure of the alternative complex III in a supercomplex with cytochrome oxidase, Nature, 557(7703):123-126.

Damian M, Pons V, Renault P, M'Kadmi C, Delort B, Hartmann L, Kaya AI, Louet M, Gagne D, Ben Haj Salah K, Denoyelle S, Ferry G, Boutin JA, Wagner R, Fehrentz JA, Martinez J, Marie J, Floquet N, Galès C, Mary S, Hamm HE, Banères JL. GHSR-D2R heteromerization modulates dopamine signaling through an effect on G protein conformation. Proc Natl Acad Sci USA, 115(17):4501-4506

Stroud Z, Hall SCL, Dafforn TR. Purification of membrane proteins free from conventional detergents: SMA, new polymers, new opportunities and new insights. Methods. pii: S1046-2023(17)30488-7.

Grethen A, Glueck D, Keller S. Role of Coulombic Repulsion in Collisional Lipid Transfer Among SMA(2:1)-Bounded Nanodiscs. J Membr Biol. 251(3):443-451.

Liang B, Tamm LK. Solution NMR of SNAREs, complexin and α-synuclein in association with membrane-mimetics. Prog Nucl Magn Reson Spect. 105, 41-53.

Hardy D, Desuzinges Mandon E, Rothnie A, Jawhari A. The yin and yang of solubilization and stabilization for wild-type and full-length membrane protein. Methods. pii: S1046-2023(17)30434-6.

Skrzypek R, Iqbal S, Callaghan R. Methods of reconstitution to investigate membrane protein function. Methods. pii: S1046-2023(17)30446-2.

Calabrese AN, Radford SE. Mass spectrometry-enabled structural biology of membrane proteins. Methods. pii: S1046-2023(17)30373-0.

Maher J, Allen M. Planar lipid bilayers in recombinant ion channel research. Methods. pii: S1046-2023(17)30449-8.

Penny WM, Palmer CP. Determination of lipid bilayer affinities and solvation characteristics by electrokinetic chromatography using polymer-bound lipid bilayer nanodiscs. Electrophoresis. 39(5-6):844-852.

Yasuhara K, Arakida J, Ravula T, Ramadugu SK, Sahoo B, Kikuchi JI, Ramamoorthy A. Spontaneous Lipid Nanodisc Fomation by Amphiphilic Polymethacrylate Copolymers. J Am Chem Soc. 139(51):18657-18663.

Ravula T, Hardin NZ, Ramadugu SK, Cox SJ, Ramamoorthy A. Formation of pH-Resistant Monodispersed Polymer-Lipid Nanodiscs. Angew Chem Int Ed Engl. 57(5):1342-1345.

Swainsbury DJK, Proctor MS, Hitchcock A, Cartron ML, Qian P, Martin EC, Jackson PJ, Madsen J, Armes SP, Neil Hunter C. Probing the local lipid environment of the Rhodobacter sphaeroides cytochrome bc(1) and Synechocystis sp. PCC 6803 cytochrome b(6)f complexes with styrene maleic acid. Biochim Biophys Acta. pii: S0005-2728(17)30197-4.

Oluwole AO, Klingler J, Danielczak B, Babalola JO, Vargas C, Pabst G, Keller S. Formation of Lipid-Bilayer Nanodiscs by Diisobutylene/Maleic Acid (DIBMA) Copolymer. Langmuir. 33(50):14378-14388.

Schmidt V, Sturgis JN. Modifying styrene-maleic acid co-polymer for studying lipid nanodiscs. Biochim Biophys Acta. 1860(3):777-783.

Hall SCL, Tognoloni C, Price GJ, Klumperman B, Edler KJ, Dafforn TR, Arnold T. The influence of poly(styrene-co-maleic acid) copolymer structure on the properties and self-assembly of SMALP nanodiscs. Biomacromolecules 19(3):761-772.

Esmaili M and Overduin M. Membrane biology visualized in nanometer-sized discs formed by styrene maleic acid polymers, Biochim Biophys Acta.1860(2):257-263.

Parmar M, Rawson S, Scarff CA, Goldman A, Dafforn TR, Muench SP, Postis VLG. Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure. Biochim Biophys Acta 1860(2):378-383.


Fiori MC, Jiang Y, Zheng W, Anzaldua M, Borgnia MJ, Altenberg GA & Liang H. Polymer Nanodiscs: Discoidal Amphiphilic Block Copolymer Membranes as a New Platform for Membrane Proteins. Sci Rep 7: 15227

Reading E, Hall Z, Martens C, Haghighi T, Findlay H, Ahdash Z, Politis A, Booth PJ. Interrogating membrane protein conformational dynamics within native lipid compositions. Angew Chem Int Ed Engl. 56(49):15654-15657.

Meister A, Blume A. (Cryo)Transmission Electron Microscopy of Phospholipid Model Membranes Interacting with Amphiphilic and Polyphilic Molecules. Polymers 2017, 9(10), 521.

Efremov RG, Gatsogiannis C, Raunser S. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM. Methods Enzymol. 594:1-30.

Ma F, Swainsbury DJK, Jones MR, van Grondelle R. Photoprotection through ultrafast charge recombination in photochemical reaction centres under oxidizing conditions. Philos Trans R Soc Lond B Biol Sci. 372(1730). pii: 20160378.

Smith AAA, Autzen HE, Laursen T, Wu V, Yen M, Hall A, Hansen SD, Cheng Y, Xu T. Controlling Styrene Maleic acid lipid particles through RAFT. Biomacromolecules 18(11):3706-3713.

Ramadugu VSK, Di Mauro GM, Ravula T, Ramamoorthy A. Polymer nanodiscs and macro-nanodiscs of a varying lipid composition. Chem Commun (Camb). 53(78):10824-10826.

Ravula T, Hardin NZ, Ramadugu SK, Ramamoorthy A. pH Tunable and Divalent Metal Ion Tolerant Polymer Lipid Nanodiscs. Langmuir33(40):10655-10662.

Grethen A, Oluwole AO, Danielczak B, Vargas C, Keller S. Thermodynamics of nanodisc formation mediated by styrene/maleic acid (2:1) copolymer. Sci Rep. 7(1):11517.

Pardo JJD, Dörr JM, Renne MF, Ould-Braham T, Koorengevel MC, van Steenbergen MJ, Killian JA. Thermotropic properties of phosphatidylcholine nanodiscs bounded by styrene-maleic acid copolymers. Chem Phys Lipids. pii: S0009-3084(17)30155-X.

Pollock NL, Lee SC, Patel JH, Gulamhussein AA, Rothnie AJ. Structure and function of membrane proteins encapsulated in a polymer-bound lipid bilayer. Biochim Biophys Acta. pii: S0005-2736(17)30262-6

Dörr JM, van Coevorden-Hameete MH, Hoogenraad CC, Antoinette Killian J. Solubilization of human cells by the styrene-maleic acid copolymer: Insights from fluorescence microscopy. Biochim Biophys Acta. 1859(11):2155-2160.

Fiori MC, Jiang Y, Altenberg GA, Liang H. Polymer-encased nanodiscs with improved buffer compatibility. Sci Rep. 7(1):7432.

Swainsbury DJK, Scheidelaar S, Foster N, van Grondelle R, Killian JA, Jones MR. The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes. Biochim Biophys Acta. 1859(10):2133-2143.

Ravula T, Ramadugu SK, Di Mauro G, Ramamoorthy A. Bioinspired, Size-Tunable Self-Assembly of Polymer-Lipid Bilayer Nanodiscs. Angew Chem Int Ed Engl. 56(38):11466-11470.

Denisov IG, Sligar SG. Nanodiscs in Membrane Biochemistry and Biophysics. Chem Rev. 117(6):4669-4713.

Stetsenko A, Albert Guskov A. An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization. Crystals 7, 197.

Huang J, Turner SR. Recent advances in alternating copolymers: The synthesis, modification, and applications of precision polymers. Polymer 116, 572-586.

Dowall JS, Ntai I, Hake J, Whitley PR, Mason JM, Pudney CR, Brown DR. Steady-State Kinetics of α-Synuclein Ferrireductase Activity Identifies the Catalytically Competent Species. Biochemistry. 56(19):2497-2505.

Hu Z, Ho JC, Nallani M. Synthetic (polymer) biology (membrane): functionalization of polymer scaffolds for membrane proteins. Curr Opin Biotechnol. 46:51-56.

Bos I, Bland KM, Tian L, Croce R, Frankel LK, van Amerongen H, Bricker TM, Wientjes E. Multiple LHCII antennae can transfer energy efficiently to a single Photosystem I. Biochim Biophys Acta. 1858(5):371-378.

Zoghbi ME, Altenberg GA. Membrane protein reconstitution in nanodiscs for luminescence spectroscopy studies. Nanotech Rev 6: 1

Penny WM, Steele HB, Ross JB, Palmer CP. Phospholipid bilayer affinities and solvation characteristics by electrokinetic chromatography with a nanodisc pseudostationary phase. Electrophoresis. 38(5):738-746.

Beales PA, Khan S, Muench SP, Jeuken LJ. Durable vesicles for reconstitution of membrane proteins in biotechnology. Biochem Soc Trans. 45(1):15-26.

Sahu ID, Zhang R, Dunagan MM, Craig AF, Lorigan GA. Characterization of KCNE1 inside Lipodisq Nanoparticles for EPR Spectroscopic Studies of Membrane Proteins. J Phys Chem B. 121(21):5312-5321.

Zhang R, Sahu ID, Bali AP, Dabney-Smith C, Lorigan GA. Characterization of the structure of lipodisq nanoparticles in the presence of KCNE1 by dynamic light scattering and transmission electron microscopy. Chem Phys Lipids. 203:19-23.

Cuevas Arenas R, Danielczak B, Martel A, Porcar L, Breyton C, Ebel C, Keller S. Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs. Sci Rep. 5;7:45875.

Puthenveetil R, Nguyen K, Vinogradova O. Nanodiscs and Solution NMR: preparation, application and challenges. Nanotechnol Rev. 6(1):111-126.

Rehan S, Paavilainen VO, Jaakola VP. Functional reconstitution of human equilibrative nucleoside transporter-1 into styrene maleic acid co-polymer lipid particles. Biochim Biophys Acta. 1859(5):1059-1065.

Bersch B, Dörr JM, Hessel A, Killian JA, Schanda P. Proton-Detected Solid-State NMR Spectroscopy of a Zinc Diffusion Facilitator Protein in Native Nanodiscs. Angew Chem Int Ed Engl. 56(9):2508-2512.

Oluwole AO, Danielczak B, Meister A, Babalola JO, Vargas C, Keller S. Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene / Maleic Acid Copolymer. Angew Chem Int Ed Engl. 6;56(7):1919-1924.

Broecker J, Eger BT, Ernst OP. Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs. Structure 25, 1–9.

Dominguez Pardo JJ, Dörr JM, Iyer A, Cox RC, Scheidelaar S, Koorengevel MC, Subramaniam V, Killian JA. Solubilization of lipids and lipid phases by the styrene-maleic acid copolymer. Eur Biophys J. 46(1):91-101.


D. V. Bagrova et al, Characterization of Lipodisc Nanoparticles Containing Sensory Rhodopsin II and Its Cognate Transducer from Natronomonas pharaonis. Biophysics 61(6): 942–949.

Rawson S, Davies S, Lippiat JD, Muench SP. The changing landscape of membrane protein structural biology through developments in electron microscopy, Molecular Membrane Biology, 33:1-2, 12-22

Komar J, Alvira S, Schulze RJ, Martin R, Lycklama A Nijeholt JA, Lee SC, Dafforn TR, Deckers-Hebestreit G, Berger I, Schaffitzel C, Collinson I. Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC. Biochem J. 473(19):3341-54.

Viegas A, Viennet T, Etzkorn M. The power, pitfalls and potential of the nanodisc system for NMR-based studies. Biol Chem. 397(12):1335-1354.

Grewal YS, Shiddiky MJ, Mahler SM, Cangelosi GA, Trau M. Nanoyeast and Other Cell Envelope Compositions for Protein Studies and Biosensor Applications. ACS Appl Mater Interfaces. 8(45):30649-30664.

Laursen T, Borch J, Knudsen C, Bavishi K, Torta F, Martens HJ, Silvestro D, Hatzakis NS, Wenk MR, Dafforn TR, Olsen CE, Motawia MS, Hamberger B, Møller BL, Bassard JE. Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Science. 354(6314):890-893.

Scheidelaar S, Koorengevel MC, van Walree CA, Dominguez JJ, Dörr JM, Killian JA. Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers. Biophys J. 111(9):1974-1986.

Craig AF, Clark EE, Sahu ID, Zhang R, Frantz ND, Al-Abdul-Wahid MS, Dabney-Smith C, Konkolewicz D, Lorigan GA. Tuning the size of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for biophysical studies. Biochim Biophys Acta. 1858(11):2931-2939.

Parmar MJ, Lousa Cde M, Muench SP, Goldman A, Postis VL. Artificial membranes for membrane protein purification, functionality and structure studies. Biochem Soc Trans. 44(3):877-82.

Hazell G, Arnold T, Barker RD, Clifton LA, Steinke NJ, Tognoloni C, Edler KJ. Evidence of Lipid Exchange in Styrene Maleic Acid Lipid Particle (SMALP) Nanodisc Systems. Langmuir 15;32(45):11845-11853.

Morrison KA, Akram A, Mathews A, Khan ZA, Patel JH, Zhou C, Hardy DJ, Moore-Kelly C, Patel R, Odiba V, Knowles T, Javed MU, Chmel NP, Dafforn TR, Rothnie AJ. Membrane protein extraction and purification using styrene-maleic acid (SMA) co-polymer: Effect of variations in polymer structure. Biochem J 1;473(23):4349-4360.

Rajesh S, Overduin M, Bonev BB. NMR of Membrane Proteins: Beyond Crystals. Adv Exp Med Biol. 922:29-42.

Rothnie AJ. Detergent-Free Membrane Protein Purification. Methods Mol Biol. 1432:261-7.

Lee SC, Pollock NL. Membrane proteins: is the future disc shaped? Biochem Soc Trans. 44(4):1011-8.

Smirnova IA, Sjöstrand D, Li F, Björck M, Schäfer J, Östbye H, Högbom M, von Ballmoos C, Lander GC, Ädelroth P, Brzezinski P. Isolation of yeast complex IV in native lipid nanodiscs. Biochim Biophys Acta. 1858(12):2984-2992.

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Michael Overduin submits Nanodisks for Drug Screening to Birmingham Research and Development Ltd, and is awarded a Technology Transfer Fund Grant by the Central Technology Belt for commercial development of the SMALP system with Steve Tonge being a co-applicant.


A Royal Society Wolfson Research Merit Award to Michael Overduin entitled Structural Biology of Membrane Spanning Proteins by NMR Spectroscopy is funded from 2004-2009.