SMALP network
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Commercial SMA Polymers

The discovery that copolymers composed of styrene and maleic acid groups can solubilize native lipid:protein complexes direct from cells or raw membrane has stimulated a flurry of new research.

Cube Biotech offers a range of SMALP products that have been widely used and are recommended to test and optimize purification of membrane proteins along with bound lipids and partner proteins.

Product S:MA Ratio† Mw† (g/mole) Source
SMALP 140 1.4:1  5 kDa Aurorium and Cube Biotech
SMALP 140-I 1.4:1 5 kDa Aurorium and Cube Biotech
SMALP 200 2:1 6.5 kDa Aurorium and Cube Biotech
SMALP 300 3:1 10 kDa Aurorium and Cube Biotech
SMALP BZ25 25% 5.5 kDa Cube Biotech
SMALP BZ30 30% 5.5 kDa Cube Biotech
SMALP BZ35 35% 5.5 kDa Cube Biotech
SMALP BZ40 40% 5.5 kDa Cube Biotech
SMA-EA 1.1:1 7.8 kDa Anatrace and Molecular Dimensions
SMA-ED 1.1:1 7.8 kDa

Anatrace and Molecular Dimensions
SMA-QA 1.1:1 9.0 kDa Anatrace and Molecular Dimensions
SMAd-A 1.1:1 7.8 kDa Anatrace and Molecular Dimensions
zSMA-116 1:1 20-27 kDa Anatrace

Contact Cube Biotech for ordering SMALP reagents as well as Synthetic Nanodisc Screening Kit MAXI and Synthetic Nanodisc Screening Kit MINI, which include 17 or 8 polymer types, respectively.

† The SMA copolymers are defined by the average ratio of styrene to maleic acid repeat units (S:MA). The lengths and blockiness of the polymers is different for each. The number average molecular weight (Mn) is total weight of the polymer molecules divided by the number of molecules. The weight average molecular weight (Mw) is more sensitive to the larger chains present. The polydispersity index (PDI) equals Mw/Mn and is a measure of distribution of the molecular weights.

Related polymers that form native nanodiscs

In addition to SMA polymers, related synthetic copolymers convert membranes into discs and are listed below.

Product Subunit Ratio† Mw† (g/mole) Mn† (g/mole) Source
AASTY 11-45 45-55% 10-11 kDa   Cube Biotech
AASTY 11-50 50% 10-11 kDa   Cube Biotech
AASTY 11-55 55-45% 10-11 kDa   Cube Biotech
AASTY 6-45 45-55% 5-6 kDa   Cube Biotech
AASTY 6-50 50% 5-6 kDa   Cube Biotech
AASTY 6-55 55-45% 5-6 kDa   Cube Biotech
DIBMA 10 , HEPES 50% ~10 kDa   Cube Biotech
DIBMA 10 , TRIS 50% ~10 kDa   Cube Biotech
DIBMA 12 , HEPES 50% ~12 kDa   Cube Biotech
DIBMA 12, TRIS 50% ~12 kDa   Cube Biotech
DIBMA Glusoamine, HEPES 50% 10-12 kDa   Cube Biotech
DIBMA Glusoamine, TRIS 50% 10-12 kDa   Cube Biotech
DIBMA Glycerol, HEPES 50% ~10 kDa   Cube Biotech
DIBMA Glycerol, TRIS 50% ~10 kDa   Cube Biotech
DIBMA Screening Kit, HEPES       Cube Biotech
DIBMA Screening Kit, TRIS       Cube Biotech
Glyco-DIBMA 50% 15701 g/Mol   Cube Biotech
Sulfo-DIBMA 50% ~8 kDa   Cube Biotech
Ultrasolute Amphipol 17 50%     Cube Biotech
Ultrasolute Amphipol 18 50%     Cube Biotech
Amphipol A8-35 50% ~9 kDa   Anatrace
BMA101-DIBMA 50% 15300 8400 Anatrace
DIBMA 50%   Glycon
Glyco-DIBMA 50%   Glycon
PMA 1:1.1     Avanti

Notes:

SMALP 140, 200 and 300 reagents are produced by Aurorium and sold through Cube Biotech.

SMALP-BZ reagents were developed by Cube Biotech in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa; they are synthesized via RAFT polymerization to generate a better defined polymer size and a low dispersity.

DIBMA is based upon the work of Sandro Keller. Anatrace and Cube Biotech are offering ready-to-use DIBMA products in two buffer systems as well as protocols and services. Sokalan CP9 is the tradename of an alternating copolymer of maleic acid and diisobutylene (2,4,4-trimethylpent-1-ene) (DIBMA).

PMA refers to polymethacrylate copolymer product "Nāˆ’C4āˆ’52āˆ’6.9" from Avanti. This belongs to the family of polymers in Prof. A Ramamoorthy's JACS paper in 2017.

Ultrasolute Amphipols were developed by Dr. Manuela Zoonens and colleagues at CNRS, France as published in Anal Chem and Biomacromolecules in 2022

Figure: Polymers developed by the research community for native nanodisc production. 

We welcome you to discuss your thoughts on SMA and related polymers below or by contacting us.