SMALP publications and patents since 2009

The following papers report developments in the SMALP field:


Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Böttcher B. The MscS-like channel YnaI has a gating mechanism based on flexible pore helices. PNAS USA. 117(46):28754-2876 .

Korotych OI, Nguyen TT, Reagan BC, Burch-Smith TM, Bruce BD. Poly(styrene-co-maleic acid)-mediated isolation of supramolecular membrane protein complexes from plant thylakoids. BBA Bioenerg. 148347.

Lavington S, Watts A. Lipid nanoparticle technologies for the study of G protein-coupled receptors in lipid environments. Biophys Rev. in press. 

 Reis RI, Moraes I. Probing Membrane Protein Assembly into Nanodiscs by In Situ Dynamic Light Scattering: A2A Receptor as a Case Study. Biology (Basel). in press.

Marty MT. Nanodiscs and Mass Spectrometry: Making Membranes Fly. Int J Mass Spectrom. 458:116436

 Ueta T, Kojima K, Hino T, Shibata M, Nagano S, Sudo Y. Applicability of Styrene-Maleic Acid Copolymer for Two Microbial Rhodopsins, RxR and HsSRI. Biophys J. S0006-3495(20)30737-2.

Bernhard M, Laube B. Thermophoretic analysis of ligand-specific conformational states of the inhibitory glycine receptor embedded in copolymer nanodiscs. Sci Rep. 10(1):16569.

Overduin M, Esmaili M. Functional derivatives of maleimide copolymers for nanodisc production. PCT/US20/57069

de Jonge PA, Smit Sibinga DJC, Boright OA, Costa AR, Nobrega FL, Brouns SJJ, Dutilh BE. Development of styrene maleic acid lipid particles (SMALPs) as a tool for studies of phage-host interactions. J Virol. in press.

Schachter I, Allolio C, Khelashvili G, Harries D. Confinement in Nanodiscs Anisotropically Modifies Lipid Bilayer Elastic Properties. J Phys Chem B. 124(33):7166-7175.

Ravula T, Sahoo BR, Dai X, Ramamoorthy A. Natural-abundance 17O NMR spectroscopy of magnetically aligned lipid nanodiscs. Chem Commun. 56(69):9998-10001.

Medina-Carmona E , Varela L , Hendry AC , Thompson GS , White LJ , Boles JE , Hiscock JR , Ortega-Roldan JL . A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chem Commun. 56(78):11665-11668.

Guo R, Sumner J, Qian IS.Diisobutylene Maleic Acid Copolymer (DIBMA) Lipid Particle: A “Stealth” Membrane Mimetic for Neutron Scattering. ChemRXiv.

AAA Smith, HE Autzen, B Faust, JL Mann, BW Muir, Howard S, Postma A, Spakowitz AJ, Cheng Y, Appel EA, Lipid Nanodiscs via Ordered Copolymers, Chem. in press

Harwood C, Sykes DA, Hoare B, Heydenreich FM, Uddin R, Poyner DR, Briddon SJ, Veprintsev DB, Functional Solubilisation of the Β2-Adrenoceptor (Β 2AR) Using Diisobutylene Maleic Acid (DIBMA). iScience

Hothersall JD, Jones AY, Dafforn TR, Perrior T, Chapman KL. Releasing the technical 'shackles' on GPCR drug discovery: opportunities enabled by detergent-free polymer lipid particle (PoLiPa) purification. Drug Discov Today. S1359-6446(20)30337-8.

Hardin N. Synthesis and Characterization of Polymers, and Development of Polymer Based Lipid-Nanodiscs. PhD thesis.

Centola G, Deredge DJ, Hom K, Ai Y, Dent AT, Xue F, Wilks A. Gallium(III)-Salophen as a Dual Inhibitor of Pseudomonas aeruginosa Heme Sensing and Iron AcquisitionACS Infect Dis. 2020;6(8):2073-2085.

Hrmova M, Gilliham M, Tyerman SD. Plant transporters involved in combating boron toxicity: beyond 3D structures. Biochem Soc Trans 48(4): 1683–1696.

Flores JA, Haddad BG, Dolan KA, Myers JB, Yoshioka CC, Copperman J, Zuckerman DM, Reichow SL. Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å. Nat Commun. 11(1):4331.

Lemieux MJ, Overduin M. Structure and function of proteins in membranes and nanodiscs. BBA Biomembr. in press.

Lavington S, Watts A. Detergent-free solubilisation & purification of a G protein coupled receptor using a polymethacrylate polymer, BBA Biomembr. in press.

Esmaili M, Brown CJ, Shaykhutdinov R, Acevedo-Morantes C, Wang YL, Wille H, Gandour RD, Turner SR, Overduin M. Homogeneous nanodiscs of native membranes formed by stilbene-maleic-acid copolymers. Nanoscale. 12(32):16705-16709.

Ayub H, Clare M, Milic I, Chmel NP, Böning H, Devitt A, Krey T, Bill RM, Rothnie AJ. CD81 extracted in SMALP nanodiscs comprises two distinct protein populations within a lipid environment enriched with negatively charged headgroups. BBA Biomembr183419.

Cunningham RD, Kopf AH, Elenbaas BOW, Staal BBP, Pfukwa R, Killian JA, Klumperman B. Iterative RAFT-Mediated Copolymerization of Styrene and Maleic Anhydride toward Sequence- and Length-Controlled Copolymers and Their Applications for Solubilizing Lipid Membranes. Biomacromolecules 21(8):3287-3300.

Marconnet A, Michon B, Le Bon C, Giusti F, Tribet C, Zoonens M. Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers. Biomacromolecules 21(8):3459-3467.

Johnson RM, Fais C, Parmar M, Cheruvara H, Marshall RL, Hesketh SJ, Feasey MC, Ruggerone P, Vargiu AV, Postis VLG, Muench SP, Bavro VN. Cryo-EM Structure and Molecular Dynamics Analysis of the Fluoroquinolone Resistant Mutant of the AcrB Transporter from Salmonella. Microorganisms 8(6):943.

Cleary SP , Prell JS . Distinct classes of multi-subunit heterogeneity: analysis using Fourier Transform methods and native mass spectrometry. Analyst145(13):4688-4697.

Pellowe GA, Findlay HE, Lee K, Gemeinhardt TM, Blackholly LR, Reading E, Booth PJ. Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies. Biochemistry. 59(30):2764-2775.

Torgersen ML , Judge PJ, Juarez JFB, Pandy AD, Fusser M, Davies CW, Maciejewska MK, Yin DJ, Mælandsmo GM, Skotland T, Watts A, Sandvig K. Physicochemical characterization, toxicity and in vivo biodistribution studies of a discoidal, lipid-based drug delivery vehicle: Lipodisq nanoparticles containing doxorubicin. J Biomed Nanotechnol. 16(4):419-431.

Sahu ID, Lorigan GA. Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins. Biomolecules 2020, 10(5), 763

Fiori MC, Zheng W, Kamilar E, Simiyu G, Altenberg GA, Liang H. Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers. Sci Rep. 10(1):9940.

Krishnarjuna B, Ravula T , Ramamoorthy A . Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids. Chem Commun. 56(48):6511-6514.

Olerinyova A, Sonn-Segev A, Gault J, Eichmann C, Schimpf J, Kopf AH, Rudden LSP, Ashkinadze D, Bomba R, Greenwald J, Degiacomi MT, Killian JA, Friedrich T, Riek R, Struwe WB, Kukura P. Mass Photometry of Membrane Proteins, bioRxiv.

Dodd R, Schofield DJ, Wilkinson T, Britton ZT. Generating therapeutic monoclonal antibodies to complex multi-spanning membrane targets: Overcoming the antigen challenge and enabling discovery strategies. Methods. S1046-2023(19)30296-8.

Pellowe GA, Booth PJ. Structural insight into co-translational membrane protein folding. BBA Biomembr. 1862(1):183019.

Mutalik SP, Pandey A, Mutalik S. Nanoarchitectronics: A versatile tool for deciphering nanoparticle interaction with cellular proteins, nucleic acids and phospholipids at biological interfaces. Int J Biol Macromol. 151:136-158.

Raltchev KP. NMR structural studies of full-length Bcl-xL in nanodiscs, PhD thesis.

Adão R, Cruz PF, Vaz DC, Fonseca F, Pedersen JN, Ferreira-da-Silva F, Brito RMM, Ramos CHI, Otzen D, Keller S, Bastos M. DIBMA nanodiscs keep α-synuclein folded. BBA Biomembr. 1862(9):183314.

Esmaili M, Acevedo-Morantes C, Wille H, Overduin M. The effect of hydrophobic alkyl sidechains on size and solution behaviors of nanodiscs formed by alternating styrene maleamic copolymer. BBA Biomembr. 1862(10):183360.

Yoder N, Gouaux E. Conserved His-Gly motif of acid-sensing ion channels resides in a reentrant ‘loop’ implicated in gating and ion selectivity, Elife 9:e56527.

Grime RL, Goulding J, Uddin R, Stoddart LA, Hill SJ, Poyner DR, Briddon SJ, Wheatley M. Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles. Nanoscale. 12(21):11518-11525

Di Mauro GM, Hardin NZ, Ramamoorthy A. Lipid-nanodiscs formed by paramagnetic metal chelated polymer for fast NMR data acquisition. BBA Biomembr. 1862(9):183332

Danielczak B, Keller S. Lipid Exchange among Polymer-Encapsulated Nanodiscs by Time-Resolved Förster Resonance Energy Transfer Methods. 180:27-34.

Park SH, Wu J, Yao Y, Singh C, Tian Y, Marassi FM, Opella SJ. Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy. BBA Biomembr. 1862(9):183333.

Esmaili M, Tancowny BP, Wang X, Moses A, Cortez L, Sim V, Wille H, Overduin M. Native nanodiscs formed by styrene-maleic acid copolymer derivatives help recover infectious prion multimers bound to brain-derived lipidsJ Biol Chem. 295(25):8460-8469.

Yu J, Zhu H, Lape R, Greiner T, Shahoei R, Wang Y, Du J, Lü W, Tajkhorshid E, Sivilotti L, Gouaux E. Mechanism of gating and partial agonist action in the glycine receptor. bioRxiv in press.

Hall SCL, Clifton LA, Tognoloni C, Morrison KA, Knowles TJ, Kinane CJ, Dafforn TR, Edler KJ, Arnold T. Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer. J Colloid Interface Sci. 574:272‐284.

van't Klooster JS, Cheng TY, Sikkema HR, Jeucken A, Moody B, Poolman B. Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteinsElife. 9:e57003.

Angiulli G, Dhupar HS, Suzuki H, Wason IS, Duong Van Hoa F, Walz T. New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteinsElife. 9:e53530.

Lloris-Garcerá P, Klinter S, Chen L, Skynner MJ, Löving R, Frauenfeld J. DirectMX - One-Step Reconstitution of Membrane Proteins From Crude Cell Membranes Into Salipro NanoparticleFront Bioeng Biotechnol: 8:215

Gulamhussein AA, Uddin R, Tighe BJ, Poyner DR, Rothnie AJ. A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification. BBA Biomembr. 1862(7):183281.

Tanaka M, Miyake H, Oka S, Maeda S, Iwasaki K, Mukai T. Effects of charged lipids on the physicochemical and biological properties of lipid-styrene maleic acid copolymer discoidal particlesBBA Biomembr. 1862(5):183209.

Phan MD, Korotych OI, Brady NG, Davis MM, Satija SK, Ankner JF, Bruce BD. X-ray and Neutron Reflectivity Studies of Styrene-Maleic Acid Copolymer Interactions with Galactolipid-Containing Monolayers. Langmuir. 36, 14, 3970–3980

Campuzano IDG, Nshanian M, Spahr C, Lantz C, Netirojjanakul C, Li H, Wongkongkathep P, Wolff JJ, Loo JA. High Mass Analysis with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: From Inorganic Salt Clusters to Antibody Conjugates and Beyond. J Am Soc Mass Spectrom. 31, 5, 1155–1162.

Sahu ID, Dixit G, Reynolds WD, Kaplevatsky R, Harding BD, Jaycox CK, McCarrick RM, Lorigan GA. Characterization of the Human KCNQ1 Voltage Sensing Domain (VSD) in Lipodisq Nanoparticles for Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of Membrane Proteins. J Phys Chem B. 124, 12, 2331–2342.

Barniol-Xicota M., and Verhelst, SHL. Comparative analysis of maleic acid copolymer-based lipid nanodiscs reveals preferential lipid and protein solubilization, chemRxiv.

Olerinyova A, Sonn-Segev A, Gault J, Eichmann C, Schimpf J, Kopf AH, Rudden LSP, Ashkinadze D, Bomba R, Greenwald J, Degiacomi MT, Killian A, Friedrich T, Riek RR, Struwe WB, Kukura P, Mass Photometry of Membrane Proteins, bioRxiv.

Nakatani Y, Shimaki Y, Dutta D, Muench SP, Ireton K, Cook GM, Jeuken LJC. Unprecedented Properties of Phenothiazines Unraveled by a NDH-2 Bioelectrochemical Assay Platform. J Am Chem Soc. 142(3):1311-1320.

Burridge KM, Harding BD, Sahu ID, Kearns MM, Stowe RB, Dolan MT, Edelmann RE, Dabney-Smith C, Page RC, Konkolewicz D, Lorigan GA. Simple Derivatization of RAFT-Synthesized Styrene-Maleic Anhydride Copolymers for Lipid Disk Formulations. Biomacromolecules 21, 3, 1274-1284.

Routledge SJ, Jamshad M, Little HA, Lin YP, Simms J, Thakker A, Spickett CM, Bill RM, Dafforn TR, Poyner DR,Wheatley M. Ligand-induced conformational changes in a SMALP-encapsulated GPCR. BBA Biomembr. 1862(6):183235.

Tascón I, Sousa JS, Corey RA, Mills DJ, Griwatz D, Aumüller N, Mikusevic V, Stansfeld PJ, Vonck J, Hänelt I. Structural basis of proton-coupled potassium transport in the KUP family. Nat Commun. 11(1):626

Tanaka K, Prendergast A, Hintzen J, Kumar A, Chung M, Koleske, A Crawford, J Nicoli S, Schwartz MA. Latrophilins are essential for endothelial junctional fluid shear stress mechanotransduction, bioRxiv.

Guo, Y. Be Cautious with Crystal Structures of Membrane Proteins or Complexes Prepared in Detergents. Crystals 10(2), 86.

Azouz M, Gonin M, Fiedler S, Faherty J, Decossas M, Cullin C, Villette S, Lafleur M, Alves ID, Lecomte S, Ciaccafava A. Microfluidic diffusional sizing probes lipid nanodiscs formation. BBA Biomembr. 1862(6):183215.

Horsey AJ, Briggs DA, Holliday ND, Briddon SJ, Kerr ID. Application of fluorescence correlation spectroscopy to study substrate binding in styrene maleic acid lipid copolymer encapsulated ABCG2. BBA Biomembr. 1862(6) 183218.

Ravula T, Kim J, Lee DK, Ramamoorthy A. Magnetic Alignment of Polymer Nanodiscs Probed by Solid-State NMR Spectroscopy. Langmuir, 36, 5, 1258–1265

Tanaka M, Miyake H, Oka S, Maeda S, Iwasaki K, Mukai T. Effects of charged lipids on the physicochemical and biological properties of lipid-styrene maleic acid copolymer discoidal particles. BBA Biomembr. 1862(5): 183209

Errey JC, Fiez-Vandal C. Production of membrane proteins in industry: The example of GPCRs. Protein Expr Purif. 169:105569.

Colbasevici A, Voskoboynikova N, Orekhov PS, Bozdaganyan ME, Karlova MG, Sokolova OS, Klare JP, Mulkidjaniana A, Shaitan KV, Steinhoff HJ. Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations, BBA Biomembr, 1862(5):183207

Juarez JF, Munoz-Garcia JC, dos Reis R, A Henry A, McMillan D, Kriek M, Wood M, Vandenplas C, Sands Z, Castro L, Taylor R, Watts A, Detergent free extraction of a functional low expressing GPCR from a human cell line, BBA Biomembr, 1862(3): 183152

Barniol-Xicota M, Verhelst SH, Isolation of intramembrane proteases in membrane-like environments, BBA Biomemb1862(4):183193

Hesketh SJ, Klebl DP, Thomsen M, Higgins AJ, Pickles IB, Sobott F, Sivaprasadarao A, Postis VL, Muench SP, Styrene maleic-acid lipid particles (SMALPs) into detergent or amphipols: An exchange protocol for membrane protein characterisation, BBA Biomembr, 1862(5) 183192

Bai J, Wang J, Ravula T, Im SC, Anantharamaiah GM, Waskell L, Ramamoorthy A, Expression, Purification, and Functional Reconstitution of 19F-labeled Cytochrome b5 in Peptide Nanodiscs for NMR Studies, BBA Biomembr, 1862(5):183194

Hernando M, Orris G, Perodeau J, Lei S, Farens, F, Patel T, Stetefeld J, Nieuwkoop AJ, O'Neil J. Solution Structure and Oligomeric State of the E. coli Glycerol Facilitator, BBA Biomembr. 1862(5) 183191

Beriashvili D, Spencer NR, Dieckmann T, Overduin M, Palmer M, Characterization of multimeric daptomycin bound to lipid nanodiscs formed by calcium-tolerant styrene-maleic acid copolymer, BBA Biomembr, 862(6):183234

Dutta D, Esmaili M, Overduin M, Fliegel L. Expression and detergent free purification and reconstitution of the plant plasma membrane Na(+)/H(+) antiporter SOS1 overexpressed in Pichia pastoris. BBA Biomembr.1862(3):183111.

Juarez JFB, Muñoz-García JC, Dos Reis RI, Henry A, McMillan D, Kriek M, Wood M, Vandenplas C, Sands Z, Castro L, Taylor R, Watts A. Detergent-free extraction of a functional low-expressing GPCR from a human cell line. BBA Biomembr. 1862(3):183152

Ganapathy S, Opdam L, Hontani Y, Frehan S, Chen Q, Hellingwerf KJ, de Groot HJM, Kennis JTM, de Grip WJ. Membrane matters: The impact of a nanodisc-bilayer or a detergent microenvironment on the properties of two eubacterial rhodopsins. BBA Biomembr1862(2):183113.

Kopf AH, Dörr JM, Koorengevel MC, Antoniciello F, Jahn H, Killian JA. Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene-maleic acid copolymers. BBA Biomembr. 1862(2):183125.

Desrames A, Genetet S, Delcourt MP, Goossens D, Mouro-Chanteloup I. Detergent-free isolation of native red blood cell membrane complexes. BBA Biomembr1862(2):183126.

Swiecicki JM, Santana JT, Imperiali B. A Strategic Approach for Fluorescence Imaging of Membrane Proteins in a Native-like Environment. Cell Chem Biol. pii: S2451-9456(19)30390-3.

Haffke M, Duckely M, Bergsdorf C, Jaakolac VP, Shresth B, Development of a biochemical and biophysical suite for integral membrane protein targets: A review, Protein Expr Purif, 167, 105545.

Saourosa S, Cecchettia C, Jones A, Cameron AD, Byrne B, Strategies for successful isolation of a eukaryotic transporter, Protein Expr Purif, 167, 105545.

Gakhar S, Risbud SH, Longo ML. Structure retention of silica gel-encapsulated bacteriorhodopsin in purple membrane and in lipid nanodiscs. Colloids Surf B Biointerfaces. 186:110680

Wiseman DN, Otchere A, Patel JH, Uddin R, Pollock NL, Routledge SJ, Rothnie AJ, Slack C, Poyner DR, Bill RM, Goddard AD. Expression and purification of recombinant G protein-coupled receptors: A review. Protein Expr Purif.167, 105524.

Cherepanov DA, Brady NG, Shelaev IV, Nguyen J, Gostev FE, Mamedov MD, Nadtochenko VA, Bruce BD. PSI-SMALP, a detergent-free cyanobacterial photosystem I reveals faster femtosecond photochemistry. Biophys J. 118(2):337-351. 


Scalise M, Jaakola VP. Membrane Proteins: New Approaches to Probes, Technologies, and Drug Design. SLAS Discov. 24(9):865-866.

Hardy D, Bill RM, Rothnie AJ, Jawhari A. Stabilization of Human Multidrug Resistance Protein 4 (MRP4/ABCC4) Using Novel Solubilization Agents. SLAS Discov. 24(10):1009-1017.

Overduin M, Esmaili M. Structures and Interactions of Transmembrane Targets in Native Nanodiscs. SLAS Discovery. 24(10):943-952.

Chill JH, Qasim A, Sher I, Gross R. NMR Perspectives of the KcsA Potassium Channel in the Membrane Environment. Isr. J. Chem.59, 1001–1013.

Lee SC, Collins R, Lin YP, Jamshad M, Broughton C, Harris SA, Hanson BS, Tognoloni C, Parslow RA, Terry AE, Rodger A, Smith CJ, Edler KJ, Ford R, Roper DI, Dafforn TR. Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ. Sci Rep 9(1):18712.

Smith AJ, Wright KE, Muench SP, Schumann S, Whitehouse A, Porter KE, Colyer J. Styrene maleic acid recovers proteins from mammalian cells and tissues while avoiding significant cell death. Sci Rep. 9(1):16408.

Bibow S. Opportunities and Challenges of Backbone, Sidechain, and RDC Experiments to Study Membrane Protein Dynamics in a Detergent-Free Lipid Environment Using Solution State NMR. Front Mol Biosci. 6:103.

Bibow S. Opportunities and Challenges of Backbone, Sidechain, and RDC Experiments to Study Membrane Protein Dynamics in a Detergent-Free Lipid Environment Using Solution State NMR. Front Mol Biosci. 6:103.

Entova S, Guan Z, Imperiali B. Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates. Arch Biochem Biophys. 675:108111.

Brady NG, Li M, Ma Y, Gumbart JC, Bruce BD. Non-detergent isolation of a cyanobacterial photosystem I using styrene maleic acid alternating copolymers. RSC Adv 9, 31781-31796.

Orwick Rydmark M, Christensen MK, Köksal ES, Kantarci I, Kustanovich K, Yantchev V, Jesorka A, Gözen I. Styrene maleic acid copolymer induces pores in biomembranes. Soft Matter. 15(39):7934-7944.

Hardin NZ, Kocman V, Di Mauro GM, Ravula T, Ramamoorthy A. Metal-Chelated Polymer Nanodiscs for NMR Studies. Angew Chem Int Ed Engl. 58(48):17246-17250.

Sarkar K, Joedicke L, Westwood M, Burnley R, Wright M, McMillan D, Byrne B. Modulation of PTH1R signaling by an ECD binding antibody results in inhibition of β-arrestin 2 coupling. Sci Rep. 9(1):14432.

Autzen HE, Julius D, Cheng Y. Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment. Curr Opin Struct Biol. 58:259-268.

Puthenveetil R, Vinogradova O. Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments. J Biol Chem. 294(44):15914-15931.

Zhang Q, Cherezov V. Chemical tools for membrane protein structural biology. Curr Opin Struct Biol. 58:278-285.

R Cunningham, A Kopf, R Pfukwa, B Klumperman. Synthesis of non-alternating styrene-maleic anhydride (smanh) copolymers via raft polymerisation–investigating the efficiency of styrene-maleic acid lipid particle (smalp) formation. 13th International Conference on Advanced Polymers via Macromolecular Engineering.

Reading E. Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs. Trends Biochem Sci. 141(2): 1054-61

Shelby ML, He W, Dang AT, Kuhl TL, Coleman MA. Cell-Free Co-Translational Approaches for Producing Mammalian Receptors: Expanding the Cell-Free Expression Toolbox Using Nanolipoproteins. Front Pharmacol. 10:744.

Voskoboynikova, N., Karlova, M., Kurre, R., Heinisch, J., Steinhoff, H., Shaitan, K., & Sokolova, O. (2019). SMA-based Extraction of the Yeast Cell Wall Integrity Sensor Mid2 from Native Membranes of Saccharomyces cerevisiae – Electron Microscopy Study. Microsc Microanal, 25(S2), 1338-1339.

Almeida-Hernandez Y, Tidow H. Soluble Regions of GlpG Influence Protein–Lipid Interactions and Lipid Distribution. J. Phys. Chem. B 123(37)7852-7858.

Su CC, Morgan CE, Kambakam S, Rajavel M, Scott H, Huang W, Emerson CC, Taylor DJ, Stewart PL, Bonomo RA, Yu EW. Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump. MBio. 10(4).

Horsey, Aaron J. (2019) Analysis of ABCG2 pharmacology and oligomerisation by solution based fluorescence correlation spectroscopy. PhD thesis, University of Nottingham.

Roxlau TR, Investigation of the lipid environment of the mammalian transamidase complex, PhD Thesis Heidelberg University

Obata T. Metabolons in plant primary and secondary metabolism, Phytochemistry Reviews. Phytochem Rev, 18(6) 1483–1507

Kostelic MM, Ryan AM, Reid DJ, Noun JM, Marty MT. Expanding the Types of Lipids Amenable to Native Mass Spectrometry of Lipoprotein Complexes. J Am Soc Mass Spectrom. 30(8):1416-1425

Kehlenbeck DM, Josts I, Nitsche J, Busch S, Forsyth VT, Tidow H. Comparison of lipidic carrier systems for integral membrane proteins - MsbA as case study. Biol Chem, 400(11):1509-1518.

Redhair M, Clouser AF, Atkins WM. Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs. Chem Phys Lipids. 220:14-22

Pollock NL, Rai M, Simon KS, Hesketh SJ, Teo ACK, Parmar M, Sridhar P, Collins R, Lee SC, Stroud ZN, Bakker SE, Muench SP, Barton CH, Hurlbut G, Roper DI, Smith CJI, Knowles TJ, Spickett CM, East JM, Postis V, Dafforn TR. SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis. BBA Biomembr. 1861(8):1437-1445.

Bada Juarez JF, O'Rourke D, Judge PJ, Liu LC, Hodgkin J, Watts A. Lipodisqs for eukaryote lipidomics with retention of viability: Sensitivity and resistance to Leucobacter infection linked to C.elegans cuticle composition. Chem Phys Lipids. 222:51-58

Overduin M, Esmaili M. Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics. Appl. Sci. 2019, 9(6), 1230.

Ognjenović J, Grisshammer R, Subramaniam S. Frontiers in Cryo Electron Microscopy of Complex Macromolecular Assemblies. Annu Rev Biomed Eng. 21: 395-415.

M Yokogawa, M Fukuda, M Osawa Nanodiscs for Structural Biology in a Membranous Environment. Chem Pharm Bull. 67 321-326.

Á Deák, D Sebők, E Csapó, A Bérczi, I Dékány, Zimányi L, Janovák L. Evaluation of pH-responsive poly (styrene-co-maleic acid) copolymer nanoparticles for the encapsulation and pH-dependent release of ketoprofen and and tocopherol model drugs. Eur Polymer J. 114, 361-368

Pinwanich P, Soisungval, A.Structural Modification of Styrene Maleic Anhydride Copolymers for Plant Bioactive Compound Extraction, Key Engineering Materials 798, 351-357.

Sahoo BR, Genjo T, Moharana KC, Ramamoorthy A. Self-Assembly of Polymer-Encased Lipid Nanodiscs and Membrane Protein Reconstitution. J Phys Chem B. 123: 4562-4570.

Hortigüela V, Larrañaga E, Lagunas A, Acosta GA, Albericio F, Andilla J, Loza-Alvarez P, Martínez E. Large -Area Biomolecule Nanopatterns on Diblock Copolymer Surfaces for Cell Adhesion Studies. Nanomaterials (Basel). 9(4). pii: E579

Sun C, Gennis RB. Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs. Chem Phys Lipids. 221:114-119

Mosslehy W, Voskoboynikova N, Colbasevici A, Ricke A, Klose D, Klare JP, Mulkidzhanyan AY, Steinhoff HJ. Conformational dynamics of sensory rhodopsin II in nanolipoprotein and styrene-maleic acid lipid particles. Photochem Photobiol. 95(5):1195-1204.

Danielczak B, Meister A, Keller S. Influence of Mg(2+) and Ca(2+) on Nanodisc Formation by Diisobutylene/Maleic Acid (DIBMA) Copolymer. Chem Phys Lipids. 221: 30-38.

Kopf AH, Koorengevela MC, van Walreea CA, Dafforn TR, Killian A. A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers. Chem Phys Lipids. 218: 85-90.

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Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M. G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Biosci Rep 35 pii: e00188. PMID: 25720391.


Jamshad M, Grimard V, Idini I, Knowles TJ, Dowle MR, Schofield N, Sridhar P, Lin YP, Finka R, Wheatley M, Thomas ORT, Palmer RE, Overduin M, Govaerts C, Ruysschaert JM, Edler KJ, Dafforn TR. Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Research 8: 774-789.

Paulin S, Jamshad M, Dafforn TR, Garcia-Lara J, Foster SJ, Galley NF, Roper DI, Rosado H, Taylor PW. Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. Nanotechnology 25:285101. PMID: 24972373.

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Gulati S, Jamshad M, Knowles TJ, Morrison KA, Downing R, Cant N, Collins R, Koenderink JB, Ford RC, Overduin M, Kerr ID, Dafforn TR, Rothnie AJ. Detergent-free purification of ABC (ATP-binding-cassette) transporters. Biochem J 461:269-78. PMID: 24758594.

Sahu ID, McCarrick RM, Troxel KR, Zhang R, Smith HJ, Dunagan MM, Swartz MS, Rajan PV, Kroncke BM, Sanders CR, Lorigan GA. DEER EPR measurements for membrane protein structures via bifunctional spin labels and lipodisq nanoparticles. Biochemistry 52:6627-32. PMID: 23984855.

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Jamshad M, Lin YP, Knowles TJ, Parslow RA, Harris C, Wheatley M, Poyner DR, Bill RM, Thomas OR, Overduin M, Dafforn TR. Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem Soc Trans 39:813-8. PMID: 21599653.

Rajesh S, Knowles T, Overduin M. Production of membrane proteins without cells or detergents. N Biotechnol 28:250-4. PMID: 20654746.


Overduin M, Jamshad M, Lin Y, Knowles T, Jazayeri A, Poyner D, Bill R, Wheatley M, Dafforn T. Amphipathic polymer-based nanoparticles for purifying and characterizing stable and active GPCRs without detergents. Keystone Symposium on G Protein-Coupled Receptors.

Overduin M, Knowles T, Dafforn T. Solubilisation of membrane proteins. Patent GB2010/001309; WO2011/004158.


Knowles TJ, Finka R, Smith C, Lin YP, Dafforn T, Overduin M. Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J Am Chem Soc 131:7484-5. PMID: 19449872.


Tonge S. Compositions comprising a lipid and copolymer of styrene and maleic acid (note that the membrane protein data included in this patent is from Knowles et. al., 2009). Patent PCT/GB2006/050134; WO2006/129127.

Overduin, M, Tonge S. Memtein: Providing Membrane Protein Solutions, Venturefest finalist at the Oxford University Business Plan Competition.


Michael Overduin submits Nanodisks for Drug Screening to Birmingham Research and Development Ltd, and is awarded a Technology Transfer Fund Grant by the Central Technology Belt for commercial development of the SMALP system with Steve Tonge being a co-applicant.


A Royal Society Wolfson Research Merit Award to Michael Overduin entitled Structural Biology of Membrane Spanning Proteins by NMR Spectroscopy is funded from 2004-2009.